SLINGSHOT-1L Primary Antibody
|Formulation||Purified antibody in PBS with 0.05% sodium azide|
|Immunogen||Synthesized peptide fragment of human SLINGSHOT-1L (AA: 1032-1044 ) expressed in E. Coli.|
|Shipping Information||This product will ship in a box containing blue ice at a temperature of 4°C. Learn More|
|ICC (Immunocytochemistry)||1/200 - 1/1000|
|FCM (Flow Cytometry)||1/200 - 1/400|
Figure 1:Immunofluorescence analysis of Hela cells using SLINGSHOT-1L mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 2: Flow cytometric analysis of Hela cells using SLINGSHOT-1L mouse mAb (green) and negative control (red).
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
|Description||The protein encoded by this gene belongs to the slingshot homolog (SSH) family of phosphatases, which regulate actin filament dynamics. The SSH proteins dephosphorylate and activate the actin binding/depolymerizing factor cofilin, which subsequently binds to actin filaments and stimulates their disassembly. Cofilin is inactivated by kinases such as LIM domain kinase-1 (LIMK1), which may also be dephosphorylated and inactivated by SSH proteins. The SSH family thus appears to play a role in actin dynamics by reactivating cofilin proteins. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.|
|References (references)||Molecules. 2012 Dec 17;17(12):14975-94. |
Nan Fang Yi Ke Da Xue Xue Bao. 2011 Jun;31(6):928-32.