SLINGSHOT-1L Primary Antibody

Item Information
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Price
Description
The protein encoded by this gene belongs to the slingshot homolog (SSH) family of phosphatases, which regulate actin filament dynamics. The SSH proteins dephosphorylate and activate the actin binding/depolymerizing factor cofilin, which subsequently binds to actin filaments and stimulates their disassembly. Cofilin is inactivated by kinases such as LIM domain kinase-1 (LIMK1), which may also be dephosphorylated and inactivated by SSH proteins. The SSH family thus appears to play a role in actin dynamics by reactivating cofilin proteins. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
Product Overview
Entrez GenelD
54434
Aliases
SSH1;SSH1L
Clone#
1A5C8
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Synthesized peptide fragment of human SLINGSHOT-1L (AA: 1032-1044 ) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
Molecules. 2012 Dec 17;17(12):14975-94.
Nan Fang Yi Ke Da Xue Xue Bao. 2011 Jun;31(6):928-32.
Product Image
Immunofluorescence analysis
Figure 1:Immunofluorescence analysis of Hela cells using SLINGSHOT-1L mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 1:Immunofluorescence analysis of Hela cells using SLINGSHOT-1L mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 2: Flow cytometric analysis of Hela cells using SLINGSHOT-1L mouse mAb (green) and negative control (red).
Figure 2: Flow cytometric analysis of Hela cells using SLINGSHOT-1L mouse mAb (green) and negative control (red).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.