SKP2 Primary Antibody

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Description

SKP2: S-phase kinase-associated protein 2 (p45). This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class; in addition to an F-box, this protein contains 10 tandem leucine-rich repeats. This protein is an essential element of the cyclin A-CDK2 S-phase kinase. It specifically recognizes phosphorylated cyclin-dependent kinase inhibitor 1B (CDKN1B, also referred to as p27 or KIP1) predominantly in S phase and interacts with S-phase kinase-associated protein 1 (SKP1 or p19). In addition, this gene is established as a protooncogene causally involved in the pathogenesis of lymphomas. Alternative splicing of this gene generates 2 transcript variants encoding different isoforms.

Product Overview
Entrez GenelD
6502
Aliases
FBL1; FLB1; FBXL1; MGC1366; SKP2
Clone#
6G9D10
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of SKP2 (aa1-130) expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ELISA
1/10000
References
1. Acta Biochim Biophys Sin (Shanghai). 2007 Dec;39(12):999-1007.
2. Clin Cancer Res. 2008 Apr 1;14(7):1966-75.
Product Image
Western Blot
Figure 1: Western blot analysis using SKP2 mouse mAb against truncated Trx-SKP2 recombinant protein (1) and GST-SKP2 (aa1-130) recombinant protein (2).
Figure 1: Western blot analysis using SKP2 mouse mAb against truncated Trx-SKP2 recombinant protein (1) and GST-SKP2 (aa1-130) recombinant protein (2).
For Research Use Only. Not for use in diagnostic procedures.