SK2 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesSPHK2; SK-2; SPK 2; SPK-2
ProductOrderS
Clone#9C5E1
Entrez GenelD56848
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG1
ImmunogenSynthesized peptide of human SK2 (AA: 36-52).
MW69.2kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
Sequence
36-52
Catalog#: 30888
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Images
Elisa
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Immunofluorescence analysis
Figure 2:Immunofluorescence analysis of HeLa cells using SK2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 2:Immunofluorescence analysis of HeLa cells using SK2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Immunohistochemical analysis
Figure 3:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using SK2 mouse mAb with DAB staining.
Figure 3:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using SK2 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 4:Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues using SK2 mouse mAb with DAB staining.
Figure 4:Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues using SK2 mouse mAb with DAB staining.
Flow cytometric
Figure 5:Flow cytometric analysis of HeLa cells using SK2 mouse mAb (green) and negative control (red).
Figure 5:Flow cytometric analysis of HeLa cells using SK2 mouse mAb (green) and negative control (red).
Product Overview
DescriptionThis gene encodes one of two sphingosine kinase isozymes that catalyze the phosphorylation of sphingosine into sphingosine 1-phosphate. Sphingosine 1-phosphate mediates many cellular processes including migration, proliferation and apoptosis, and also plays a role in several types of cancer by promoting angiogenesis and tumorigenesis. The encoded protein may play a role in breast cancer proliferation and chemoresistance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
References (references)
References (references)1.Tumour Biol. 2014 Jan;35(1):363-8.
2.Electrophoresis. 2011 Jun;32(13):1742-9.