SK2 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesSPHK2; SK-2; SPK 2; SPK-2
ProductOrderS
Clone#3C8D3
Entrez GenelD56848
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG1
ImmunogenSynthesized peptide of human SK2 (AA: 36-52).
MW69.2kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
Sequence
36-52
Catalog#: 30887
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Images
Elisa
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Immunofluorescence analysis
Figure 2:Immunofluorescence analysis of HeLa cells using SK2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 2:Immunofluorescence analysis of HeLa cells using SK2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 3:Flow cytometric analysis of HeLa cells using SK2 mouse mAb (green) and negative control (red).
Figure 3:Flow cytometric analysis of HeLa cells using SK2 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 4:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using SK2 mouse mAb with DAB staining.
Figure 4:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using SK2 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 5:Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues using SK2 mouse mAb with DAB staining.
Figure 5:Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues using SK2 mouse mAb with DAB staining.
Product Overview
DescriptionThis gene encodes one of two sphingosine kinase isozymes that catalyze the phosphorylation of sphingosine into sphingosine 1-phosphate. Sphingosine 1-phosphate mediates many cellular processes including migration, proliferation and apoptosis, and also plays a role in several types of cancer by promoting angiogenesis and tumorigenesis. The encoded protein may play a role in breast cancer proliferation and chemoresistance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
References (references)
References (references)1.Tumour Biol. 2014 Jan;35(1):363-8.
2.Electrophoresis. 2011 Jun;32(13):1742-9.