SK2 Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene encodes one of two sphingosine kinase isozymes that catalyze the phosphorylation of sphingosine into sphingosine 1-phosphate. Sphingosine 1-phosphate mediates many cellular processes including migration, proliferation and apoptosis, and also plays a role in several types of cancer by promoting angiogenesis and tumorigenesis. The encoded protein may play a role in breast cancer proliferation and chemoresistance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Product Overview
Entrez GenelD
56848
Aliases
SPHK2; SK-2; SPK 2; SPK-2
Clone#
3C8D3
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Synthesized peptide of human SK2 (AA: 36-52).
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Tumour Biol. 2014 Jan;35(1):363-8.
2.Electrophoresis. 2011 Jun;32(13):1742-9.
2.Electrophoresis. 2011 Jun;32(13):1742-9.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Immunofluorescence analysis
Figure 2:Immunofluorescence analysis of HeLa cells using SK2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 3:Flow cytometric analysis of HeLa cells using SK2 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 4:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using SK2 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 5:Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues using SK2 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.