SIRT6 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesSIR2L6
ProductOrderS
Clone#2G1H1
Entrez GenelD51548
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human SIRT6 (AA: 141-250) expressed in E. Coli.
MW39kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
141-250
Catalog#: 30856
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Images
Elisa
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western Blot
Figure 2:Western blot analysis using SIRT6 mAb against human SIRT6 (AA: 141-250) recombinant protein. (Expected MW is 38 kDa)
Figure 2:Western blot analysis using SIRT6 mAb against human SIRT6 (AA: 141-250) recombinant protein. (Expected MW is 38 kDa)
Western Blot
Figure 3:Western blot analysis using SIRT6 mAb against HEK293 (1) and SIRT6 (AA: 141-250)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using SIRT6 mAb against HEK293 (1) and SIRT6 (AA: 141-250)-hIgGFc transfected HEK293 (2) cell lysate.
Immunofluorescence analysis
Figure 4:Immunofluorescence analysis of HeLa cells using SIRT6 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Figure 4:Immunofluorescence analysis of HeLa cells using SIRT6 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Immunofluorescence analysis
Figure 5:Immunofluorescence analysis of HeLa cells using SIRT6 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 5:Immunofluorescence analysis of HeLa cells using SIRT6 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Product Overview
DescriptionThis gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family. Alternative splicing results in multiple transcript variants.
References (references)
References (references)1.Mol Med Rep. 2014 Mar;9(3):882-8.
2.Carcinogenesis. 2013 Jul;34(7):1476-86.