SH2B2
Item Information
Catalog #
Size
Price
Description
The protein encoded by this gene is expressed in B lymphocytes and contains pleckstrin homology and src homology 2 (SH2) domains. In Burkitt's lymphoma cell lines, it is tyrosine-phosphorylated in response to B cell receptor stimulation. Because it binds Shc independent of stimulation and Grb2 after stimulation, it appears to play a role in signal transduction from the receptor to the Shc/Grb2 pathway.
Product Overview
Entrez GenelD
10603
Aliases
APS
Clone#
5E11G7
Host / Isotype
Mouse / Mouse IgG2b
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human SH2B2 (AA: 497-676) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Cancer Epidemiol Biomarkers Prev. 2002 Jul;11(7):664-9.
2.J Biol Chem. 2005 May 13;280(19):18943-9.
2.J Biol Chem. 2005 May 13;280(19):18943-9.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using SH2B2 mAb against human SH2B2 (AA: 497-676) recombinant protein. (Expected MW is 21.8 kDa)
Western Blot
Figure 3:Western blot analysis using SH2B2 mAb against HEK293-6e (1) and SH2B2 (AA: 497-676)-hIgGFc transfected HEK293-6e (2) cell lysate.
Immunofluorescence analysis
Figure 4:Flow cytometric analysis of HEK293 cells using SH2B2 mouse mAb (green) and negative control (red).
Immunofluorescence analysis
Figure 5:Flow cytometric analysis of HepG2 cells using SH2B2 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using SH2B2 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded colon cancer tissues using SH2B2 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.