SETDB1 Primary Antibody

Item Information
Catalog #
Size
Price
Description

This gene encodes a histone methyltransferase. The encoded enzyme catalyzes the reaction of S-adenosyl-L-methionine and histone L-lysine to produce S-adenosyl-L-homocysteine and histone N(6)-methyl-L-lysine. The encoded protein likely functions in transcriptional repression. Alternatively spliced transcript variants have been described.

Product Overview
Entrez GenelD
9869
Aliases
ESET; KG1T; KMT1E; KIAA0067; H3-K9-HMTase4; SETDB1
Clone#
5H6A12
Host / Isotype
Mouse / IgG1
Species Reactivity
Human, Mouse, Monkey
Immunogen
Purified recombinant fragment of human SETDB1 expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/200 - 1/1000
ELISA
1/10000
References
1. Proteomics. 2005 Sep;5(14):3589-99.
2. Proc Natl Acad Sci U S A. 2006 Apr 4;103(14):5308-13.
3. Mol Cell Biochem. 2007 Nov;305(1-2):35-44.
Product Image
Western Blot
Figure 1: Western blot analysis using SETDB1 mouse mAb against MCF-7 (1),T47D (2), HEK293 (3), JURKAT (4), NIH/3T3 (5), F9 (6), RAW246.7 (7) and Cos7 (8) cell lysate.
Figure 1: Western blot analysis using SETDB1 mouse mAb against MCF-7 (1),T47D (2), HEK293 (3), JURKAT (4), NIH/3T3 (5), F9 (6), RAW246.7 (7) and Cos7 (8) cell lysate.
Immunofluorescence analysis
Figure 2: Immunofluorescence analysis of LOVO cells using SETDB1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Figure 2: Immunofluorescence analysis of LOVO cells using SETDB1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
For Research Use Only. Not for use in diagnostic procedures.