SETD7 Primary Antibody
Item Information
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Price
Description
Histone methyltransferase that specifically monomethylates Lys-4 of histone H3. H3 Lys-4 methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in the transcriptional activation of genes such as collagenase or insulin. Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription. Has also methyltransferase activity toward non-histone proteins such as p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins. Monomethylates Lys-189 of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II. Monomethylates Lys-372 of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation.
Product Overview
Entrez GenelD
80854
Aliases
KMT7; SET7; SET9; SET7/9
Clone#
1B3B8
Host / Isotype
Mouse / IgG2a
Species Reactivity
Human, Rat, Monkey
Immunogen
Purified recombinant fragment of human SETD7 (AA: 107-366) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.PLoS One. 2016 May 16;11(5):e0154939.
2.Sci Rep. 2015 Oct 5;5:14368.
2.Sci Rep. 2015 Oct 5;5:14368.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using SETD7 mAb against human SETD7 (AA: 107-366) recombinant protein. (Expected MW is 55 kDa)
Western Blot
Figure 3:Western blot analysis using SETD7 mAb against HEK293 (1) and SETD7 (AA: 107-366)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using SETD7 mouse mAb against MCF-7 (1), Hela (2), A549 (3), COS7 (4), Jurkat (5), and PC-12 (6) cell lysate.
Flow cytometric
Figure 5:Flow cytometric analysis of Hela cells using SETD7 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.