Mouse Monoclonal Antibody to SCARB1
Item Information
Catalog #
Size
Price
Description
The protein encoded by this gene is a plasma membrane receptor for high density lipoprotein cholesterol (HDL). The encoded protein mediates cholesterol transfer to and from HDL. In addition, this protein is a receptor for hepatitis C virus glycoprotein E2. Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2019]
Product Overview
Entrez GenelD
949
Aliases
CLA1; SRB1; CLA-1; SR-BI; CD36L1; HDLQTL6
Clone#
5E9H10
Host / Isotype
Mouse / IgG2a
Immunogen
Purified recombinant fragment of human SCARB1 (AA: Extra(33-232)) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1,Arch Biochem Biophys. 2019 May 15;666:1-7. 2,Cancer Res. 2019 Jul 1;79(13):3320-3331.
Product Image
Elisa
![Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/4ed1593e48dcdb467670b8600218cd8da65cef0de1e515792221d343494c0567.jpg?v=1666705762)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
![Figure 2:Western blot analysis using SCARB1 mAb against human SCARB1 (AA: Extra(33-232)) recombinant protein. (Expected MW is 26kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/abcb2ae6b482effd9557acb59c14338696bd2d608364d328fa1dcc1b32c3a0cf.jpg?v=1666705762)
Figure 2:Western blot analysis using SCARB1 mAb against human SCARB1 (AA: Extra(33-232)) recombinant protein. (Expected MW is 26kDa)
Western Blot
![Figure 3:Western blot analysis using SCARB1 mAb against HEK293-6e (1) and human SCARB1 (AA: Extra(33-232))-hIgGFc transfected HEK293-6e (2) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/09b08c74f4081bbfd4049d4b52065a629113f8daea45f986185038bc29be405b.jpg?v=1666705762)
Figure 3:Western blot analysis using SCARB1 mAb against HEK293-6e (1) and human SCARB1 (AA: Extra(33-232))-hIgGFc transfected HEK293-6e (2) cell lysate.
Western Blot
![Figure 4:Western blot analysis using SCARB1 mouse mAb against Hela (1), U937 (2), and HePG2 (3) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/7cd2f77ab81d1e76c8af0487f6cae193d2f2be509f770b0ef5118cd9491a818c.jpg?v=1666705762)
Figure 4:Western blot analysis using SCARB1 mouse mAb against Hela (1), U937 (2), and HePG2 (3) cell lysate.
Flow cytometric analysis
![Figure 5:Flow cytometric analysis of BEL-7402 cells using SCARB1 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/afa3d16a894ebfe6a36fa6da8167dc4e1b0591e4fbf6024db1ef03b3f13871e4.jpg?v=1666705762)
Figure 5:Flow cytometric analysis of BEL-7402 cells using SCARB1 mouse mAb (green) and negative control (red).
Flow cytometric analysis
![Figure 6:Flow cytometric analysis of Hela cells using SCARB1 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/e4ea7f40a0890432bed3f03eef2b1fb3dacdeb6ff1c5fe33c700f111790cc75b.jpg?v=1666705762)
Figure 6:Flow cytometric analysis of Hela cells using SCARB1 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
![Figure 7:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using SCARB1 mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/76a10523c33463d2342ca535c2aa259fc9e9e0a0f7822bb5269a8b80f22301b5.jpg?v=1666705762)
Figure 7:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using SCARB1 mouse mAb with DAB staining.
Immunohistochemical analysis
![Figure 8:Immunohistochemical analysis of paraffin-embedded lung cancer tissues using SCARB1 mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/779a4d1d79abb1051477b29daa68f7c0755eb9792a34c32c660f212f9ccf6e53.jpg?v=1666705762)
Figure 8:Immunohistochemical analysis of paraffin-embedded lung cancer tissues using SCARB1 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.