SARS-CoV-2 Primary Antibody
Item Information
Catalog #
Size
Price
Description
The RBD protein encoded by S gene (part of MN908947.2) is a receptor-binding protein of coronavirus that was shown to bind ACE-2 protein. RBD protein is highly homologius to SARS RBD protein.
Product Overview
Entrez GenelD
MN908947.2
Aliases
S1
Clone#
CR3022
Host / Isotype
Mouse / Human IgG1
Immunogen
Purified recombinant fragment of SARS RBD protein.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/1000
ELISA
1/200
References
1. Xiaolong Tian, Cheng Li, Ailing Huang, Shuai Xia, Sicong Lu, Zhengli Shi, LuLu, Shibo Jiang, Zhenlin Yang, Yanling Wu, Tianlei Ying. Potent binding of 2019 novel coronavirus 1 spike protein by a SARS coronavirus-specific human monoclonal antibody. 2020. bioRxiv preprint doi: https://doi.org/10.1101/2020.01.28.923011.2. Jan ter Meulen , Edward N van den Brink, Leo L M Poon, Wilfred E Marissen, Cynthia S W Leung, Freek Cox, Chung Y Cheung, Arjen Q Bakker, Johannes A Bogaards, Els van Deventer, Wolfgang Preiser, Hans Wilhelm Doerr, Vincent T Chow, John de Kruif, Joseph S M Peiris, Jaap Goudsmit. Human Monoclonal Antibody Combination Against SARS Coronavirus: Synergy and Coverage of Escape.2006 PLoS Med , 3 (7), e237
Product Image
ELISA
Figure 1: Binding of CR3022 to RBD by ELISA. For ELISA with BSA, GFP or RBD-His mammalian proteins CR3022 Ab was 1: 200 dilution; secondary anti- human Fc-HRP 1: 5000 dilution was used.
WESTERN BLOT
Figure 2: Expression of recombinant CR3022 antibody
WESTERN BLOT
Figure 3: Binding of CR3022 to RBD and NP3 (fragment 3) of 2019nCoV proteins by Western blotting . RBD- and terminal fragment of Nucleocapsid protein (NP3) were loaded on SDS gel and BSA and GFP proteins as negative controls. Western blotting was done with CR3022 antibody 1: 1000 dilution as primary antibody, and with anti-human Fc secondary antibody 1: 10,000 dilution
For Research Use Only. Not for use in diagnostic procedures.