S100B Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene is encodes a cytoplasmic protein with three cytoskeletal-associated protein-glycine-conserved (CAP-GLY) domains that functions as a deubiquitinating enzyme. Mutations in this gene have been associated with cylindromatosis, multiple familial trichoepithelioma, and Brooke-Spiegler syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Product Overview
Entrez GenelD
6285
Aliases
NEF; S100; S100-B; S100beta
Clone#
4A5H7
Host / Isotype
Mouse / Mouse IgG1
Immunogen
Purified recombinant fragment of human S100B (AA: 1-92) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4?; -20? for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Biomed Res Int. 2018 Apr 23;2018:6954045. 2.BMC Med. 2017 Apr 12;15(1):79.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using S100B mAb against human S100B (AA: 1-92) recombinant protein. (Expected MW is 24.3 kDa)
Western Blot
Figure 3:Western blot analysis using S100B mAb against HEK293 (1) and S100B (AA: 1-92)-hIgGFc transfected HEK293 (2) cell lysate.
Flow Cytometric
Figure 4:Flow cytometric analysis of Hela cells using S100B mouse mAb (green) and negative control (red).
Immunohistochemical Analysis
Figure 5:Immunohistochemical analysis of paraffin-embedded cerebellum tissues using S100B mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.