Mouse Monoclonal Antibody to S100A9
Item Information
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Price
Description
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and altered expression of this protein is associated with the disease cystic fibrosis. This antimicrobial protein exhibits antifungal and antibacterial activity.
Product Overview
Entrez GenelD
6280
Aliases
MIF; NIF; P14; CAGB; CFAG; CGLB; L1AG; LIAG; MRP14; 60B8AG; MAC387
Clone#
1B6H10
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of human S100A9 (AA: 1-114) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Front Immunol. 2019 Sep 18;10:2243. 2.J Cell Biochem. 2019 Oct;120(10):17662-17676.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using S100A9 mAb against human S100A9 (AA: 1-114) recombinant protein. (Expected MW is 38.7 kDa)
Western Blot
Figure 3:Western blot analysis using S100A9 mAb against HEK293-6e (1) and S100A9 (AA: 1-114)-hIgGFc transfected HEK293-6e (2) cell lysate.
Flow cytometric analysis
Figure 4:Flow cytometric analysis of THP-1 cells using S100A9 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 5:Immunohistochemical analysis of paraffin-embedded human brain tissues using S100A9 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using S100A9 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.