S100A4 Primary Antibody
Item Information
Catalog #
Size
Price
Description
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified.
Product Overview
Entrez GenelD
6275
Aliases
42A; 18A2; CAPL; FSP1; MTS1; P9KA; PEL98
Clone#
4A5A7
Host / Isotype
Mouse / Mouse IgG1
Species Reactivity
Human, Mouse, Rat
Immunogen
Purified recombinant fragment of human S100A4 (AA: 2-101) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Int J Mol Sci. 2021 Apr 29;22(9):4720.
2.In Vivo. Jul-Aug 2021;35(4):2099-2106.
2.In Vivo. Jul-Aug 2021;35(4):2099-2106.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using S100A4 mAb against human S100A4 (AA: 2-101) recombinant protein. (Expected MW is 37.5 kDa)
Western Blot
Figure 3:Western blot analysis using S100A4 mAb against HEK293-6e (1) and S100A4 (AA: 2-101)-hIgGFc transfected HEK293-6e (2) cell lysate.
Immunofluorescence analysis
Figure 4:Flow cytometric analysis of Hela cells using S100A4 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 5:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using S100A4 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded human brain tissues using S100A4 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded mouse spleen tissues using S100A4 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 8:Immunohistochemical analysis of paraffin-embedded mouse kidney tissues using S100A4 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 9:Immunohistochemical analysis of paraffin-embedded rat spleen tissues using S100A4 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 10:Immunohistochemical analysis of paraffin-embedded rat kidney tissues using S100A4 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 11:Immunohistochemical analysis of paraffin-embedded rabbit brain tissues using S100A4 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 12:Immunohistochemical analysis of paraffin-embedded rabbit kidney tissues using S100A4 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.