RUNX3 Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene encodes a member of the runt domain-containing family of transcription factors. A heterodimer of this protein and a beta subunit forms a complex that binds to the core DNA sequence 5'-PYGPYGGT-3' found in a number of enhancers and promoters, and can either activate or suppress transcription. It also interacts with other transcription factors. It functions as a tumor suppressor, and the gene is frequently deleted or transcriptionally silenced in cancer. Alternative splicing results in multiple transcript variants.
Product Overview
Entrez GenelD
864
Aliases
AML2; CBFA3; PEBP2aC
Clone#
8C9B6
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human RUNX3 (AA: 294-429) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
ELISA
1/10000
References
1.Genet Mol Res. 2015 Dec 1;14(4):15505-10.
2.J Pathol. 2015 Dec;237(4):520-31.
2.J Pathol. 2015 Dec;237(4):520-31.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using RUNX3 mAb against human RUNX3 (AA: 294-429) recombinant protein. (Expected MW is 40 kDa)
Western Blot
Figure 3:Western blot analysis using RUNX3 mAb against HEK293 (1) and RUNX3 (AA: 294-429)-hIgGFc transfected HEK293 (2) cell lysate.
For Research Use Only. Not for use in diagnostic procedures.