RUNX1 Primary Antibody
Item Information
Catalog #
Size
Price
Description
Core binding factor (CBF) is a heterodimeric transcription factor that binds to the core element of many enhancers and promoters. The protein encoded by this gene represents the alpha subunit of CBF and is thought to be involved in the development of normal hematopoiesis. Chromosomal translocations involving this gene are well-documented and have been associated with several types of leukemia. Three transcript variants encoding different isoforms have been found for this gene.
Product Overview
Entrez GenelD
861
Aliases
AML1; CBFA2; EVI-1; AMLCR1; PEBP2aB; CBF2alpha; AML1-EVI-1; PEBP2alpha
Clone#
6C12A5
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human RUNX1 (AA: 237-337) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Blood. 2015 Feb 5;125(6):930-40.
2.Blood. 2015 Jun 4;125(23):3570-9.
2.Blood. 2015 Jun 4;125(23):3570-9.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using RUNX1 mAb against human RUNX1 (AA: 237-337) recombinant protein. (Expected MW is 36.9 kDa)
Western Blot
Figure 3:Western blot analysis using RUNX1 mAb against HEK293 (1) and RUNX1 (AA: 237-337)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 4:Flow cytometric analysis of K562 cells using RUNX1 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.