RRM1 Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene encodes the large and catalytic subunit of ribonucleotide reductase, an enzyme essential for the conversion of ribonucleotides into deoxyribonucleotides. A pool of available deoxyribonucleotides is important for DNA replication during S phase of the cell cycle as well as multiple DNA repair processes. Alternative splicing results in multiple transcript variants.
Product Overview
Entrez GenelD
6240
Aliases
R1; RR1; RIR1
Clone#
5F12E6
Host / Isotype
Mouse / Mouse IgG2a
Immunogen
Purified recombinant fragment of human RRM1 (AA: 541-792) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200-1/400
ELISA
1/10000
References
1.Cancer Biomark. 2018;22(4):657-667. 2.Clin Cancer Res. 2017 Sep 1;23(17):5225-5237.
Product Image
ELISA
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)
WESTERN BLOT
Figure 2: Western blot analysis using RRM1 mAb against human RRM1 (AA: 541-792) recombinant protein. (Expected MW is 31.9 kDa)
WESTERN BLOT
Figure 3: Western blot analysis using RRM1 mAb against HEK293 (1) and RRM1 (AA: 541-792)-hIgGFc transfected HEK293 (2) cell lysate.
WESTERN BLOT
Figure 4: Western blot analysis using RRM1 mouse mAb against Hela (1), Jurkat (2), HT-29 (3), and A431 (4) cell lysate.
FLOW CYTOMETRY
Figure 5: Flow cytometric analysis of Hela cells using RRM1 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.