RPTOR Primary Antibody
| Item Information | ||
|---|---|---|
| Catalog # | Size/Concentration | Price |
| Specification | |
|---|---|
| Aliases | KOG1; Mip1 |
| ProductOrder | R |
| Clone# | 5A3A4 |
| Entrez GenelD | 57521 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
| Host | Mouse |
| Isotype | IgG1 |
| Immunogen | Purified recombinant fragment of human RPTOR (AA: 874-1009) expressed in E. Coli. |
| MW | 149kDa |
| Shipping Information | This product will ship in a box containing blue ice at a temperature of 4°C. Learn More |
| Species Reactivity | Human |
| Application | |
|---|---|
| ELISA | 1/10000 |
| ICC (Immunocytochemistry) | 1/200 - 1/1000 |
| IHC_P(Immunohistochemistry) | 1/200 - 1/1000 |
| WB (Western Blot) | 1/500 - 1/2000 |
| Sequence | |
|---|---|
| 874-1009 | |
Western Blot
Figure 1: Western blot analysis using RPTOR mAb against human RPTOR (AA: 874-1009) recombinant protein. (Expected MW is 41 kDa)
Western Blot
Figure 2: Western blot analysis using RPTOR mAb against HEK293 (1) and RPTOR (AA: 874-1009)-hIgGFc transfected HEK293 (2) cell lysate.
Immunofluorescence analysis
Figure 3: Immunofluorescence analysis of HepG2 cells using RPTOR mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Secondary antibody from Fisher (Cat#: 35503)
Immunohistochemical analysis
Figure 4: Immunohistochemical analysis of paraffin-embedded muscle tissues using RPTOR mouse mAb with DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);