RPTOR Primary Antibody

Item Information

Catalog #

Size

Price

Description

This gene encodes a component of a signaling pathway that regulates cell growth in response to nutrient and insulin levels. The encoded protein forms a stoichiometric complex with the mTOR kinase, and also associates with eukaryotic initiation factor 4E-binding protein-1 and ribosomal protein S6 kinase. The protein positively regulates the downstream effector ribosomal protein S6 kinase, and negatively regulates the mTOR kinase. Multiple transcript variants encoding different isoforms have been found for this gene.

Product Overview

Entrez GenelD

57521

Aliases

KOG1; Mip1

Clone#

5A3A4

Host / Isotype

Mouse / IgG1

Species Reactivity

Human

Immunogen

Purified recombinant fragment of human RPTOR (AA: 874-1009) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide.

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200 - 1/1000

ICC (Immunocytochemistry)

1/200 - 1/1000

ELISA

1/10000

References

1. PLoS Genet. 2010 Oct 28;6(10):e1001178.
2. Cell Cycle. 2011 Sep 15;10(18):3140-52.

Product Image

Western Blot

Figure 1: Western blot analysis using RPTOR mAb against human RPTOR (AA: 874-1009) recombinant protein. (Expected MW is 41 kDa)

Western Blot

Figure 2: Western blot analysis using RPTOR mAb against HEK293 (1) and RPTOR (AA: 874-1009)-hIgGFc transfected HEK293 (2) cell lysate.

Immunofluorescence analysis

Figure 3: Immunofluorescence analysis of HepG2 cells using RPTOR mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Secondary antibody from Fisher (Cat#: 35503)

Immunohistochemical analysis