RPTOR Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesKOG1; Mip1
ProductOrderR
Clone#5A3A4
Entrez GenelD57521
FormulationPurified antibody in PBS with 0.05% sodium azide.
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human RPTOR (AA: 874-1009) expressed in E. Coli.
MW149kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
IHC_P(Immunohistochemistry)1/200 - 1/1000
WB (Western Blot)1/500 - 1/2000
Sequence
874-1009
Catalog#: 30652
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Images
Western Blot
Figure 1: Western blot analysis using RPTOR mAb against human RPTOR (AA: 874-1009) recombinant protein. (Expected MW is 41 kDa)
Figure 1: Western blot analysis using RPTOR mAb against human RPTOR (AA: 874-1009) recombinant protein. (Expected MW is 41 kDa)
Western Blot
Figure 2: Western blot analysis using RPTOR mAb against HEK293 (1) and RPTOR (AA: 874-1009)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 2: Western blot analysis using RPTOR mAb against HEK293 (1) and RPTOR (AA: 874-1009)-hIgGFc transfected HEK293 (2) cell lysate.
Immunofluorescence analysis
Figure 3: Immunofluorescence analysis of HepG2 cells using RPTOR mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Secondary antibody from Fisher (Cat#: 35503)
Figure 3: Immunofluorescence analysis of HepG2 cells using RPTOR mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Secondary antibody from Fisher (Cat#: 35503)
Immunohistochemical analysis
Figure 4: Immunohistochemical analysis of paraffin-embedded muscle tissues using RPTOR mouse mAb with DAB staining.
Figure 4: Immunohistochemical analysis of paraffin-embedded muscle tissues using RPTOR mouse mAb with DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Product Overview
Description

This gene encodes a component of a signaling pathway that regulates cell growth in response to nutrient and insulin levels. The encoded protein forms a stoichiometric complex with the mTOR kinase, and also associates with eukaryotic initiation factor 4E-binding protein-1 and ribosomal protein S6 kinase. The protein positively regulates the downstream effector ribosomal protein S6 kinase, and negatively regulates the mTOR kinase. Multiple transcript variants encoding different isoforms have been found for this gene.

References (references)
References (references)1. PLoS Genet. 2010 Oct 28;6(10):e1001178.
2. Cell Cycle. 2011 Sep 15;10(18):3140-52.