ROCK1 Primary Antibody
|Aliases||ROCK-I; PRO0435; MGC43611; P160ROCK; MGC131603|
|Formulation||Purified antibody in PBS with 0.05% sodium azide|
|Immunogen||Purified recombinant fragment of human ROCK1 expressed in E. Coli. |
|Shipping Information||This product will ship in a box containing blue ice at a temperature of 4°C. Learn More|
|WB (Western Blot)||1/500 - 1/2000|
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Figure 1: Western blot analysis using ROCK1 mAb against human ROCK1 (AA: 403-610) recombinant protein. (Expected MW is 158 kDa)
Figure 2: Western blot analysis using ROCK1 mAb against HEK293 (1) and ROCK1 (AA: 403-610)-hIgGFc transfected HEK293 (2) cell lysate.
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity.
|References (references)||1. Am J Physiol Heart Circ Physiol. 2009 Mar;296(3):H893-9. |
2. Cancer Metastasis Rev. 2009 Jun;28(1-2):65-76.