ROCK1 Primary Antibody
Item Information
Catalog #
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Price
Description
This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity.
Product Overview
Entrez GenelD
6093
Aliases
ROCK-I; PRO0435; MGC43611; P160ROCK; MGC131603
Clone#
1H4
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human ROCK1 expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ELISA
1/10000
References
1. Am J Physiol Heart Circ Physiol. 2009 Mar;296(3):H893-9.
2. Cancer Metastasis Rev. 2009 Jun;28(1-2):65-76.
2. Cancer Metastasis Rev. 2009 Jun;28(1-2):65-76.
Product Image
Western Blot
Figure 1: Western blot analysis using ROCK1 mAb against human ROCK1 (AA: 403-610) recombinant protein. (Expected MW is 158 kDa)
Western Blot
Figure 2: Western blot analysis using ROCK1 mAb against HEK293 (1) and ROCK1 (AA: 403-610)-hIgGFc transfected HEK293 (2) cell lysate.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.