RISP

Item Information

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Size

Price

Description

UQCRFS1 (Ubiquinol-Cytochrome C Reductase, Rieske Iron-Sulfur Polypeptide 1) is a Protein Coding gene. Diseases associated with UQCRFS1 include Mitochondrial Complex Iii Deficiency, Nuclear Type 10 and Lactic Acidosis. Among its related pathways are Pathways of neurodegeneration - multiple diseases and Cardiac muscle contraction. Gene Ontology (GO) annotations related to this gene include oxidoreductase activity and 2 iron, 2 sulfur cluster binding.

Product Overview

Entrez GenelD

7386

Aliases

RIP1; RIS1; RISP; UQCR5; MC3DN10

Clone#

1D6D11

Host / Isotype

Mouse / Mouse IgG2a

Species Reactivity

Human

Immunogen

Purified recombinant fragment of human RISP (AA: 79-274) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

4℃; -20℃ for long term storage

Product Applications

WB (Western Blot)

1/500 - 1/2000

ICC (Immunocytochemistry)

1/200 - 1/1000

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1,Gynecol Oncol. 2004 Apr;93(1):54-8.
2,Am J Hum Genet. 2020 Jan 2;106(1):102-111.

Product Image

Elisa

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using RISP mAb against human RISP (AA: 79-274) recombinant protein. (Expected MW is 24.5 kDa)

Western Blot

Figure 3:Western blot analysis using RISP mAb against HEK293-6e (1) and RISP (AA: 79-274)-hIgGFc transfected HEK293-6e (2) cell lysate.

Western Blot

Figure 4:Western blot analysis using RISP mouse mAb against A549 (1), Hela (2), Raji (3) and HepG2 (4) cell lysate.

Immunohistochemical analysis

Figure 5:Immunofluorescence analysis of Hela cells using RISP mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Immunofluorescence analysis