RISP Primary Antibody
Item Information
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Description
UQCRFS1 (Ubiquinol-Cytochrome C Reductase, Rieske Iron-Sulfur Polypeptide 1) is a Protein Coding gene. Diseases associated with UQCRFS1 include Mitochondrial Complex Iii Deficiency, Nuclear Type 10 and Lactic Acidosis. Among its related pathways are Pathways of neurodegeneration - multiple diseases and Cardiac muscle contraction. Gene Ontology (GO) annotations related to this gene include oxidoreductase activity and 2 iron, 2 sulfur cluster binding.
Product Overview
Entrez GenelD
7386
Aliases
RIP1; RIS1; RISP; UQCR5; MC3DN10
Clone#
4E3D10
Host / Isotype
Mouse / Mouse IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human RISP (AA: 79-274) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1,Gynecol Oncol. 2004 Apr;93(1):54-8.
2,Am J Hum Genet. 2020 Jan 2;106(1):102-111.
2,Am J Hum Genet. 2020 Jan 2;106(1):102-111.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using RISP mAb against human RISP (AA: 79-274) recombinant protein. (Expected MW is 24.5 kDa)
Western Blot
Figure 3:Western blot analysis using RISP mAb against HEK293-6e (1) and RISP (AA: 79-274)-hIgGFc transfected HEK293-6e (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using RISP mouse mAb against A549 (1), Hela (2), Raji (3)and HepG2 (4) cell lysate.
Immunohistochemical analysis
Figure 5:Immunofluorescence analysis of Hela cells using RISP mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Immunofluorescence analysis
Figure 6:Flow cytometric analysis of Hela cells using RISP mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.