Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 2:Western blot analysis using RISP mAb against human RISP (AA: 79-274) recombinant protein. (Expected MW is 24.5 kDa)
Figure 3:Western blot analysis using RISP mAb against HEK293-6e (1) and RISP (AA: 79-274)-hIgGFc transfected HEK293-6e (2) cell lysate.
Figure 4:Western blot analysis using RISP mouse mAb against A549 (1), Hela (2), Raji (3)and HepG2 (4) cell lysate.
Figure 5:Immunofluorescence analysis of Hela cells using RISP mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 6:Flow cytometric analysis of Hela cells using RISP mouse mAb (green) and negative control (red).