RBP4 Primary Antibody
Item Information
Catalog #
Size
Price
Description
This protein belongs to the lipocalin family and is the specific carrier for retinol (vitamin A alcohol) in the blood. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin which prevents its loss by filtration through the kidney glomeruli. A deficiency of vitamin A blocks secretion of the binding protein posttranslationally and results in defective delivery and supply to the epidermal cells. (provided by RefSeq)
Product Overview
Entrez GenelD
5950
Aliases
RBP4
Clone#
4C2
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human RBP expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1. Diabetologia. 2008 Aug;51(8):1423-8.
2. J Clin Endocrinol Metab. 2008 Aug;93(8):3142-8.
2. J Clin Endocrinol Metab. 2008 Aug;93(8):3142-8.
Product Image
Western Blot
Figure 1: Western blot analysis using RBP4 mAb against HEK293 (1) and RBP4(AA: 1-201)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemical analysis
Figure 2: Immunohistochemical analysis of paraffin-embedded liver cancer tissues (left) and stomach cancer tissues (right) using RBP4 mouse mAb with DAB staining.
Immunofluorescence analysis
Figure 3: Immunofluorescence analysis of HepG2 cells using RBP4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric
Figure 4: Flow cytometric analysis of HepG2 cells using RBP4 mouse mAb (green) and negative control (purple).
Elisa
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
For Research Use Only. Not for use in diagnostic procedures.