RBL2 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesRb2; P130
ProductOrderR
Clone#2C8A8
Entrez GenelD5934
FormulationPurified antibody in PBS with 0.05% sodium azide.
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human RBL2 (AA: 939-1139) expressed in E. Coli.
MW128.4kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
939-1139
Catalog#: 30695
Inquire
Contact us to order
Images
Western Blot
Figure 1: Western blot analysis using RBL2 mAb against human RBL2 (AA: 939-1139) recombinant protein. (Expected MW is 48.7 kDa)
Figure 1: Western blot analysis using RBL2 mAb against human RBL2 (AA: 939-1139) recombinant protein. (Expected MW is 48.7 kDa)
Western Blot
Figure 2: Western blot analysis using RBL2 mAb against HEK293 (1) and RBL2 (AA: 939-1139)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 2: Western blot analysis using RBL2 mAb against HEK293 (1) and RBL2 (AA: 939-1139)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 3: Flow cytometric analysis of Hela cells using RBL2 mouse mAb (green) and negative control (red).
Figure 3: Flow cytometric analysis of Hela cells using RBL2 mouse mAb (green) and negative control (red).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Product Overview
Description

RBL2 is a Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. Binds to cyclins A and E. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. May act as a tumor suppressor.

References (references)
References (references)1. Malays J Pathol. 2009 Jun;31(1):53-6.
2. Int J Colorectal Dis. 2009 Nov;24(11):1303-10.