RBL2 Primary Antibody
Item Information
Catalog #
Size
Price
Description
RBL2 is a Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. Binds to cyclins A and E. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. May act as a tumor suppressor.
Product Overview
Entrez GenelD
5934
Aliases
Rb2; P130
Clone#
2C8A8
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human RBL2 (AA: 939-1139) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide.
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1. Malays J Pathol. 2009 Jun;31(1):53-6.
2. Int J Colorectal Dis. 2009 Nov;24(11):1303-10.
2. Int J Colorectal Dis. 2009 Nov;24(11):1303-10.
Product Image
Western Blot
Figure 1: Western blot analysis using RBL2 mAb against human RBL2 (AA: 939-1139) recombinant protein. (Expected MW is 48.7 kDa)
Western Blot
Figure 2: Western blot analysis using RBL2 mAb against HEK293 (1) and RBL2 (AA: 939-1139)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 3: Flow cytometric analysis of Hela cells using RBL2 mouse mAb (green) and negative control (red).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.