RBFOX3 Primary Antibody

Item Information

Catalog #

Size

Price

Description

RBFOX3 (RNA Binding Protein, Fox-1 Homolog (C. Elegans) 3) is a Protein Coding gene. Diseases associated with RBFOX3 include vertebral artery occlusion and tuberculum sellae meningioma. Among its related pathways are Neuroscience. GO annotations related to this gene include nucleic acid binding and nucleotide binding. An important paralog of this gene is RBFOX1.

Product Overview

Entrez GenelD

146713

Aliases

FOX3; NEUN; FOX-3; HRNBP3

Clone#

5A4B9

Host / Isotype

Mouse / IgG1

Species Reactivity

Human, Mouse

Immunogen

Purified recombinant fragment of human RBFOX3 (AA: 1-140) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

4°C; -20°C for long term storage

Product Applications

WB (Western Blot)

1/500 - 1/2000

ICC (Immunocytochemistry)

1/200 - 1/1000

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1.Neurosci Lett. 2014 Jan 13;558:97-102.
2.PLoS One. 2013 Sep 6;8(9):e73323.

Product Image

Elisa

Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using RBFOX3 mAb against human *** (AA: 1-140) recombinant protein. (Expected MW is 41.4 kDa)

Western Blot

Figure 3:Western blot analysis using RBFOX3 mAb against HEK293 (1) and RBFOX3 (AA: 1-140)-hIgGFc transfected HEK293 (2) cell lysate.

Western Blot

Figure 4:Western blot analysis using RBFOX3 mouse mAb against C2C12 (1), RAW264.7 (2), and U251 (3) cell lysate.

Immunofluorescence analysis

Figure 5:Immunofluorescence analysis of Hela cells using RBFOX3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric