RBFOX2 Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene is one of several human genes similar to the C. elegans gene Fox-1. This gene encodes an RNA binding protein that is thought to be a key regulator of alternative exon splicing in the nervous system and other cell types. The protein binds to a conserved UGCAUG element found downstream of many alternatively spliced exons and promotes inclusion of the alternative exon in mature transcripts. The protein also interacts with the estrogen receptor 1 transcription factor and regulates estrogen receptor 1 transcriptional activity. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Product Overview
Entrez GenelD
23543
Aliases
RTA; fxh; FOX2; RBM9; Fox-2; HNRBP2; HRNBP2; dJ106I20.3
Clone#
1E7G10
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human RBFOX2 (AA: 1-145) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Mol Cell Biol. 2013 Jan;33(2):396-405.
2.Sci Rep. 2016 Aug 3;6:30896.
2.Sci Rep. 2016 Aug 3;6:30896.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using RBFOX2 mAb against human RBFOX2 (AA: 1-145) recombinant protein. (Expected MW is 41.9 kDa)
Western Blot
Figure 3:Western blot analysis using RBFOX2 mAb against HEK293 (1) and RBFOX2 (AA: 1-145)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 4:Flow cytometric analysis of Jurkat cells using RBFOX2 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 5:Immunohistochemical analysis of paraffin-embedded brain tissues using RBFOX2 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.