RASSF1 Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene encodes a protein similar to the RAS effector proteins. Loss or altered expression of this gene has been associated with the pathogenesis of a variety of cancers, which suggests the tumor suppressor function of this gene. The inactivation of this gene was found to be correlated with the hypermethylation of its CpG-island promoter region. The encoded protein was found to interact with DNA repair protein XPA. The protein was also shown to inhibit the accumulation of cyclin D1, and thus induce cell cycle arrest. Several alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
Product Overview
Entrez GenelD
11186
Aliases
123F2; RDA32; NORE2A; RASSF1A; REH3P21
Clone#
4G9A3
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human RASSF1 expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Cancer Res. 2012 May 1;72(9):2206-17.
2.Cancer Biomark. 2011-2012;10(1):13-26.
2.Cancer Biomark. 2011-2012;10(1):13-26.
Product Image
Western Blot
Figure 1: Western blot analysis using RASSF1 mouse mAb against HepG2 (1) and Jurkat (2) cell lysate.
Flow cytometric
Figure 2: Flow cytometric analysis of HepG2 cells using RASSF1 mouse mAb (green) and negative control (purple).
Immunohistochemical analysis
Figure 3: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using RASSF1 mouse mAb with DAB staining.
Elisa
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
For Research Use Only. Not for use in diagnostic procedures.