RAP1GAP Primary Antibody

Item Information
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Price
Description

This gene encodes a type of GTPase-activating-protein (GAP) that down-regulates the activity of the ras-related RAP1 protein. RAP1 acts as a molecular switch by cycling between an inactive GDP-bound form and an active GTP-bound form. The product of this gene, RAP1GAP, promotes the hydrolysis of bound GTP and hence returns RAP1 to the inactive state whereas other proteins, guanine nucleotide exchange factors (GEFs), act as RAP1 activators by facilitating the conversion of RAP1 from the GDP- to the GTP-bound form. In general, ras subfamily proteins, such as RAP1, play key roles in receptor-linked signaling pathways that control cell growth and differentiation. RAP1 plays a role in diverse processes such as cell proliferation, adhesion, differentiation, and embryogenesis. Alternative splicing results in multiple transcript variants encoding distinct proteins.

Product Overview
Entrez GenelD
5909
Aliases
RAPGAP; RAP1GA1; RAP1GAP1; RAP1GAPII
Clone#
2G7B6
Host / Isotype
Mouse / IgG2b
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human RAP1GAP (AA: 412-660) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide.
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1. Endocr Relat Cancer. 2012 Jul 22;19(4):575-88.
2. Cancer Lett. 2012 Jul 1;320(1):65-71.
Product Image
Western Blot
Figure 1: Western blot analysis using RAP1GAP mAb against human RAP1GAP (AA: 412-678) recombinant protein. (Expected MW is 53.9 kDa)
Figure 1: Western blot analysis using RAP1GAP mAb against human RAP1GAP (AA: 412-678) recombinant protein. (Expected MW is 53.9 kDa)
Western Blot
Figure 2: Western blot analysis using RAP1GAP mAb against HEK293 (1) and RAP1GAP (AA: 412-678)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 2: Western blot analysis using RAP1GAP mAb against HEK293 (1) and RAP1GAP (AA: 412-678)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 3: Flow cytometric analysis of A431 cells using RAP1GAP mouse mAb (green) and negative control (red).
Figure 3: Flow cytometric analysis of A431 cells using RAP1GAP mouse mAb (green) and negative control (red).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.