RAP1A Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesRAP1; KREV1; KREV-1; SMGP21
ProductOrderR
Clone#5F8
Entrez GenelD5906
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human RAP1A expressed in E. Coli.
MW21kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
28-180
Catalog#: 30354
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Images
Western Blot
Figure 1: Western blot analysis using RAP1A mAb against human RAP1A (AA: 28-180) recombinant protein. (Expected MW is 43.2 kDa)
Figure 1: Western blot analysis using RAP1A mAb against human RAP1A (AA: 28-180) recombinant protein. (Expected MW is 43.2 kDa)
Western Blot
Figure 2: Western blot analysis using RAP1A mAb against HEK293 (1) and RAP1A (AA: 28-180)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 2: Western blot analysis using RAP1A mAb against HEK293 (1) and RAP1A (AA: 28-180)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 3: Flow cytometric analysis of A431 cells using RAP1A mouse mAb (green) and negative control (red).
Figure 3: Flow cytometric analysis of A431 cells using RAP1A mouse mAb (green) and negative control (red).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Product Overview
Description

The product of this gene belongs to the family of RAS-related proteins. These proteins share approximately 50% amino acid identity with the classical RAS proteins and have numerous structural features in common. The most striking difference between RAP proteins and RAS proteins resides in their 61st amino acid: glutamine in RAS is replaced by threonine in RAP proteins. The product of this gene counteracts the mitogenic function of RAS because it can interact with RAS GAPs and RAF in a competitive manner. Two transcript variants encoding the same protein have been identified for this gene.

References (references)
References (references)Mol Biol Cell. 2009 Apr;20(7):1916-25.
J Cell Sci. 2009 Aug 15;122(Pt 16):2996-3004.