RALA Primary Antibody
The product of this gene belongs to the small GTPase superfamily, Ras family of proteins. GTP-binding proteins mediate the transmembrane signaling initiated by the occupancy of certain cell surface receptors. This gene encodes a low molecular mass ras-like GTP-binding protein that shares about 50% similarity with other ras proteins.
Purified antibody in PBS with 0.05% sodium azide
4°C; -20°C for long term storage
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Figure 2:Western blot analysis using RALA mAb against human RALA (AA: 71-203) recombinant protein. (Expected MW is 41.5 kDa)
Figure 3:Western blot analysis using RALA mAb against HEK293 (1) and RALA (AA: 71-203)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Western blot analysis using RALA mouse mAb against HepG2 (1), MCF-7 (2), A549 (3), K562 (4), Raji (5), MOLT4 (6), Hela (7), COS7 (8), and NIH3T3 (9) cell lysate.
Figure 5:Immunofluorescence analysis of Hela cells using RALA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 6:Flow cytometric analysis of MCF-7 cells using RALA mouse mAb (green) and negative control (red).
Figure 7:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using RALA mouse mAb with DAB staining.
Figure 8:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using RALA mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.