RAG2 Primary Antibody

Item Information
Catalog #
Size
Price
Description

This gene encodes a protein that is involved in the initiation of V(D)J recombination during B and T cell development. This protein forms a complex with the product of the adjacent recombination activating gene 1, and this complex can form double-strand breaks by cleaving DNA at conserved recombination signal sequences. The recombination activating gene 1 component is thought to contain most of the catalytic activity, while the N-terminal of the recombination activating gene 2 component is thought to form a six-bladed propeller in the active core that serves as a binding scaffold for the tight association of the complex with DNA. A C-terminal plant homeodomain finger-like motif in this protein is necessary for interactions with chromatin components, specifically with histone H3 that is trimethylated at lysine 4. Mutations in this gene cause Omenn syndrome, a form of severe combined immunodeficiency associated with autoimmune-like symptoms.

Product Overview
Entrez GenelD
5897
Aliases
RAG-2; RAG2
Clone#
4D5
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human RAG2(350-527aa) expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ELISA
1/10000
References
1. J Biol Chem. 2004 Sep 10;279(37):38360-8.
2. Immunity. 2005 Aug;23(2):203-12.
3. J Clin Invest. 2010 Apr 1;120(4):1337-44. doi: 10.1172/JCI41305.
Product Image
Western Blot
Figure 1: Western blot analysis using RAG2 mouse mAb against RAG2(AA: 350-527)-hIgGFc transfected HEK293 (1)cell lysate.
Figure 1: Western blot analysis using RAG2 mouse mAb against RAG2(AA: 350-527)-hIgGFc transfected HEK293 (1)cell lysate.
For Research Use Only. Not for use in diagnostic procedures.