RAG2 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesRAG-2; RAG2
ProductOrderR
Clone#4D5
Entrez GenelD5897
FormulationAscitic fluid containing 0.03% sodium azide.
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human RAG2(350-527aa) expressed in E. Coli.
MW59kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
WB (Western Blot)1/500 - 1/2000
Sequence
350-527
Catalog#: 30031
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Images
Western Blot
Figure 1: Western blot analysis using RAG2 mouse mAb against RAG2(AA: 350-527)-hIgGFc transfected HEK293 (1)cell lysate.
Figure 1: Western blot analysis using RAG2 mouse mAb against RAG2(AA: 350-527)-hIgGFc transfected HEK293 (1)cell lysate.
Product Overview
Description

This gene encodes a protein that is involved in the initiation of V(D)J recombination during B and T cell development. This protein forms a complex with the product of the adjacent recombination activating gene 1, and this complex can form double-strand breaks by cleaving DNA at conserved recombination signal sequences. The recombination activating gene 1 component is thought to contain most of the catalytic activity, while the N-terminal of the recombination activating gene 2 component is thought to form a six-bladed propeller in the active core that serves as a binding scaffold for the tight association of the complex with DNA. A C-terminal plant homeodomain finger-like motif in this protein is necessary for interactions with chromatin components, specifically with histone H3 that is trimethylated at lysine 4. Mutations in this gene cause Omenn syndrome, a form of severe combined immunodeficiency associated with autoimmune-like symptoms.

References (references)
References (references)1. J Biol Chem. 2004 Sep 10;279(37):38360-8.
2. Immunity. 2005 Aug;23(2):203-12.
3. J Clin Invest. 2010 Apr 1;120(4):1337-44. doi: 10.1172/JCI41305.