RAG1 Primary Antibody
Item Information
Catalog #
Size
Price
Description
The protein encoded by this gene is involved in activation of immunoglobulin V-D-J recombination. The encoded protein is involved in recognition of the DNA substrate, but stable binding and cleavage activity also requires RAG2. Defects in this gene can be the cause of several diseases.
Product Overview
Entrez GenelD
5896
Aliases
RAG-1; RNF74; MGC43321
Clone#
1D9
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human RAG1 (AA: 818-868) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
ELISA
1/10000
References
1.PLoS One. 2011;6(5):e20475. 2.Immunol Lett. 2011 May;136(2):156-62.
Product Image
Western Blot
Figure 1: Western blot analysis using RAG1 mAb against human RAG1 recombinant protein. (Expected MW is 31.6 kDa)
Western Blot
Figure 2: Western blot analysis using RAG1 mAb against HEK293 (1) and RAG1 (AA: 818-868)-hIgGFc transfected HEK293 (2) cell lysate.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.