RAF1 Primary Antibody

Item Information
Catalog #
Size
Price
Description

This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.

Product Overview
Entrez GenelD
5894
Aliases
NS5; CRAF; Raf-1; c-Raf
Clone#
4G4
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human RAF1 expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
Cancer Res. 2009 Aug 15;69(16):6515-21.
Cancer Biol Ther. 2009 Sep;8(18):1729-36.
Product Image
Western Blot
Figure 1: Western blot analysis using RAF1 mAb against human RAF1 (AA: 198-407) recombinant protein. (Expected MW is 48.6 kDa)
Figure 1: Western blot analysis using RAF1 mAb against human RAF1 (AA: 198-407) recombinant protein. (Expected MW is 48.6 kDa)
Western Blot
Figure 2: Western blot analysis using RAF1 mouse mAb against HeLa (1), A431 (2), HepG (3), and SW620 (4)cell lysate.
Figure 2: Western blot analysis using RAF1 mouse mAb against HeLa (1), A431 (2), HepG (3), and SW620 (4)cell lysate.
Immunohistochemical analysis
Figure 3: Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using RAF1 mouse mAb with DAB staining.
Figure 3: Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using RAF1 mouse mAb with DAB staining.
Flow cytometric
Figure 4: Flow cytometric analysis of MCF-7 cells using RAF1 mouse mAb (green) and negative control (red).
Figure 4: Flow cytometric analysis of MCF-7 cells using RAF1 mouse mAb (green) and negative control (red).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.