RAD18 Primary Antibody
Item Information
Catalog #
Size
Price
Description
The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens.
Product Overview
Entrez GenelD
56852
Aliases
RNF73
Clone#
2G8C10
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human RAD18 (AA: 332-430) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Pigment Cell Melanoma Res. 2012 Mar;25(2):213-8.
2.J Exp Clin Cancer Res. 2009 Jul 25;28:106.
2.J Exp Clin Cancer Res. 2009 Jul 25;28:106.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western Blot
Figure 2:Western blot analysis using RAD18 mAb against human RAD18 (AA: 332-430) recombinant protein. (Expected MW is 37.3 kDa)
Western Blot
Figure 3:Western blot analysis using RAD18 mAb against HEK293 (1) and RAD18 (AA: 332-430)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 4:Flow cytometric analysis of Hela cells using RAD18 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.