RAD18 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesRNF73
ProductOrderR
Clone#2G8C10
Entrez GenelD56852
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human RAD18 (AA: 332-430) expressed in E. Coli.
MW56.2kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
332-430
Catalog#: 30832
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Images
Elisa
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western Blot
Figure 2:Western blot analysis using RAD18 mAb against human RAD18 (AA: 332-430) recombinant protein. (Expected MW is 37.3 kDa)
Figure 2:Western blot analysis using RAD18 mAb against human RAD18 (AA: 332-430) recombinant protein. (Expected MW is 37.3 kDa)
Western Blot
Figure 3:Western blot analysis using RAD18 mAb against HEK293 (1) and RAD18 (AA: 332-430)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using RAD18 mAb against HEK293 (1) and RAD18 (AA: 332-430)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 4:Flow cytometric analysis of Hela cells using RAD18 mouse mAb (green) and negative control (red).
Figure 4:Flow cytometric analysis of Hela cells using RAD18 mouse mAb (green) and negative control (red).
Product Overview
DescriptionThe protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens.
References (references)
References (references)1.Pigment Cell Melanoma Res. 2012 Mar;25(2):213-8.
2.J Exp Clin Cancer Res. 2009 Jul 25;28:106.