Rab8 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesMEL; RAB8A
ProductOrderR
Clone#7G8B11
Entrez GenelD4218
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG2b
ImmunogenPurified recombinant fragment of human Rab8 (AA: 68-207) expressed in E. Coli.
MW23.7kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
68-207
Catalog#: 30852
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Images
Elisa
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western Blot
Figure 2:Western blot analysis using Rab8 mAb against human Rab8 (AA: 68-207) recombinant protein. (Expected MW is 42 kDa)
Figure 2:Western blot analysis using Rab8 mAb against human Rab8 (AA: 68-207) recombinant protein. (Expected MW is 42 kDa)
Western Blot
Figure 3:Western blot analysis using Rab8 mAb against HEK293 (1) and Rab8 (AA: 68-207)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using Rab8 mAb against HEK293 (1) and Rab8 (AA: 68-207)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 4:Flow cytometric analysis of HeLa cells using Rab8 mouse mAb (green) and negative control (red).
Figure 4:Flow cytometric analysis of HeLa cells using Rab8 mouse mAb (green) and negative control (red).
Product Overview
DescriptionThe protein encoded by this gene is a member of the RAS superfamily which are small GTP/GDP-binding proteins with an average size of 200 amino acids. The RAS-related proteins of the RAB/YPT family may play a role in the transport of proteins from the endoplasmic reticulum to the Golgi and the plasma membrane. This protein shares 97%, 96%, and 51% similarity with the dog RAB8, mouse MEL, and mouse YPT1 proteins, respectively and contains the 4 GTP/GDP-binding sites that are present in all the RAS proteins. The putative effector-binding site of this protein is similar to that of the RAB/YPT proteins. However, this protein contains a C-terminal CAAX motif that is characteristic of many RAS superfamily members but which is not found in YPT1 and the majority of RAB proteins. Although this gene was isolated as a transforming gene from a melanoma cell line, no linkage between MEL and malignant melanoma has been demonstrable. This oncogene is located 800 kb distal to MY09B on chromosome 19p13.1.
References (references)
References (references)1.Intervirology. 2008;51(3):182-8.
2.Mol Biol Cell. 2007 Jan;18(1):47-56.