Rab5a Primary Antibody
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Description
RAB5A (RAB5A, Member RAS Oncogene Family) is a Protein Coding gene. Diseases associated with RAB5A include borna disease and choroideremia. Among its related pathways are Ras signaling pathway and Endocytosis. GO annotations related to this gene include GTP binding and GDP binding. An important paralog of this gene is RAB5C.
Product Overview
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western Blot
Figure 2:Western blot analysis using Rab5a mAb against human Rab5a (AA: 1-215) recombinant protein. (Expected MW is 49.5 kDa)
Western Blot
Figure 3:Western blot analysis using Rab5a mAb against HEK293 (1) and Rab5a (AA: 1-215)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using Rab5a mouse mAb against K562 (1), Hela (2), Jurkat (3), and HepG2 (4) cell lysate.
Immunofluorescence analysis
Figure 5:Immunofluorescence analysis of Hela cells using Rab5a mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 6:Flow cytometric analysis of Hela cells using Rab5a mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using Rab5a mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.