Rab5a Primary Antibody

Item Information

Catalog #

Size

Price

Description

RAB5A (RAB5A, Member RAS Oncogene Family) is a Protein Coding gene. Diseases associated with RAB5A include borna disease and choroideremia. Among its related pathways are Ras signaling pathway and Endocytosis. GO annotations related to this gene include GTP binding and GDP binding. An important paralog of this gene is RAB5C.

Product Overview

Entrez GenelD

5868

Aliases

RAB5

Clone#

2E8B11

Host / Isotype

Mouse / IgG1

Species Reactivity

Human

Immunogen

Purified recombinant fragment of human Rab5a (AA: 1-215) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200 - 1/1000

ICC (Immunocytochemistry)

1/200 - 1/1000

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1.Cancer Sci. 2011 Dec;102(12):2172-8.
2.Cell Mol Life Sci. 2011 Aug;68(16):2785-95.

Product Image

Elisa

Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

Western Blot

Figure 2:Western blot analysis using Rab5a mAb against human Rab5a (AA: 1-215) recombinant protein. (Expected MW is 49.5 kDa)

Western Blot

Figure 3:Western blot analysis using Rab5a mAb against HEK293 (1) and Rab5a (AA: 1-215)-hIgGFc transfected HEK293 (2) cell lysate.

Western Blot

Figure 4:Western blot analysis using Rab5a mouse mAb against K562 (1), Hela (2), Jurkat (3), and HepG2 (4) cell lysate.

Immunofluorescence analysis

Figure 5:Immunofluorescence analysis of Hela cells using Rab5a mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric