RAB27A Primary Antibody
Item Information
Catalog #
Size
Price
Description
The protein encoded by this gene belongs to the small GTPase superfamily, Rab family. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Mutations in this gene are associated with Griscelli syndrome type 2. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified.
Product Overview
Entrez GenelD
5873
Aliases
GS2; RAM; RAB27; HsT18676
Clone#
7D7C9
Host / Isotype
Mouse / IgG2b
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human RAB27A (AA: FULL(1-221)) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide.
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ELISA
1/10000
References
1. PLoS One. 2012;7(7):e41160.
2. J Biol Chem. 2011 Feb 18;286(7):5375-82.
2. J Biol Chem. 2011 Feb 18;286(7):5375-82.
Product Image
Western Blot
Figure 1: Western blot analysis using RAB27A mAb against human RAB27A (AA: FULL(1-221)) recombinant protein. (Expected MW is 50.8 kDa)
Western Blot
Figure 2: Western blot analysis using RAB27A mAb against HEK293 (1) and RAB27A (AA: FULL(1-221))-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemical analysis
Figure 3: Immunohistochemical analysis of paraffin-embedded prostate cancer tissues using RAB27A mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 4: Immunohistochemical analysis of paraffin-embedded liver cancer tissues using RAB27A mouse mAb with DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.