Rab25 Primary Antibody
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Description
Members of the Ras-related superfamily of GTP binding proteins, which includes Ras, Rho, Rab and ARF subfamilies, exhibit 30-50% similarity with Ras p21. Rab proteins play an important role for either in endocytosis or in biosynthetic protein transport. The possibility that Rab proteins might also direct the exocytosis from secretory vesicles to the plasma membrane is supported by the observation that in yeast, the SEC4 protein, which is 40% similar to Rab proteins, is associated with secretory vesicles.
Rab proteins located on the cytoplasmic face of organelles and vesicles, rab proteins are involved in intracellular membrane fusion reactions. Rab25 was cloned from a gastric parietal cell cDNA library and is expressed in epithelial tissues such as the gastrointestinal mucosae, kidney, and lung, which encoded a protein of 28 kDa
Rab proteins located on the cytoplasmic face of organelles and vesicles, rab proteins are involved in intracellular membrane fusion reactions. Rab25 was cloned from a gastric parietal cell cDNA library and is expressed in epithelial tissues such as the gastrointestinal mucosae, kidney, and lung, which encoded a protein of 28 kDa
Product Overview
Entrez GenelD
57111
Aliases
CATX-8; RAB25
Clone#
3F12F3
Species Reactivity
Human
Immunogen
Purified recombinant fragment of Rab25 expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ELISA
1/10000
References
1. JR Goldenring, KR Shen, HD Vaughan. et al. J. Biol. Chem,1993,268(25):18419-18422
2. Xiaoye W, Ravindra K, Jennifer N. et al. J. Biol. Chem,2000,275(37):29138-29146
2. Xiaoye W, Ravindra K, Jennifer N. et al. J. Biol. Chem,2000,275(37):29138-29146
Product Image
Western Blot
Figure 1: Western blot analysis using Rab25 mouse mAb against truncated Rab25 recombinant protein (1), human overy carcinoma(2), stomach carcinoma (3), breast carcinoma (4) tissue lysate.
Immunohistochemical analysis
Figure 2: Immunohistochemical analysis of paraffin-embedded human bladder carcinoma (A), breast carcinoma (B), esophagus carcinoma (C), skin carcinoma (D) tissue, showing cytoplasmic localization using Rab25 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.