RAB11FIP1 Primary Antibody
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Description
This gene encodes one of the Rab11-family interacting proteins (Rab11-FIPs), which play a role in the Rab-11 mediated recycling of vesicles. The encoded protein may be involved in endocytic sorting, trafficking of proteins including integrin subunits and epidermal growth factor receptor (EGFR), and transport between the recycling endosome and the trans-Golgi network. Alternative splicing results in multiple transcript variants. A pseudogene is described on the X chromosome
Product Overview
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
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Elisa

Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western Blot

Figure 2:Western blot analysis using RAB11FIP1 mAb against human RAB11FIP1 (AA: 130-271) recombinant protein. (Expected MW is 41.7 kDa)
Western Blot

Figure 3:Western blot analysis using RAB11FIP1 mAb against HEK293 (1) and RAB11FIP1 (AA: 130-271)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot

Figure 4:Western blot analysis using RAB11FIP1 mouse mAb against Raji (1), SW620 (2), A431 (3), SW480 (4), HepG2 (5), Hela (6), and NIH3T3 (7) cell lysate.
Immunofluorescence analysis

Figure 5:Immunofluorescence analysis of Hela cells using RAB11FIP1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Immunohistochemical analysis

Figure 6:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using RAB11FIP1 mouse mAb with DAB staining.
Immunohistochemical analysis

Figure 7:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using RAB11FIP1 mouse mAb with DAB staining.
Flow cytometric

Figure 8:Flow cytometric analysis of Raji cells using RAB11FIP1 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.