RAB11FIP1 Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene encodes one of the Rab11-family interacting proteins (Rab11-FIPs), which play a role in the Rab-11 mediated recycling of vesicles. The encoded protein may be involved in endocytic sorting, trafficking of proteins including integrin subunits and epidermal growth factor receptor (EGFR), and transport between the recycling endosome and the trans-Golgi network. Alternative splicing results in multiple transcript variants. A pseudogene is described on the X chromosome
Product Overview
Entrez GenelD
80223
Aliases
RCP; NOEL1A; rab11-FIP1
Clone#
1A12B9D8
Host / Isotype
Mouse / IgG1
Species Reactivity
Human, Mouse
Immunogen
Purified recombinant fragment of human RAB11FIP1 (AA: 130-271) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.J Clin Invest. 2009 Aug;119(8):2171-83.
2.J Mol Endocrinol. 2005 Oct;35(2):357-72.
2.J Mol Endocrinol. 2005 Oct;35(2):357-72.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western Blot
Figure 2:Western blot analysis using RAB11FIP1 mAb against human RAB11FIP1 (AA: 130-271) recombinant protein. (Expected MW is 41.7 kDa)
Western Blot
Figure 3:Western blot analysis using RAB11FIP1 mAb against HEK293 (1) and RAB11FIP1 (AA: 130-271)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using RAB11FIP1 mouse mAb against Raji (1), SW620 (2), A431 (3), SW480 (4), HepG2 (5), Hela (6), and NIH3T3 (7) cell lysate.
Immunofluorescence analysis
Figure 5:Immunofluorescence analysis of Hela cells using RAB11FIP1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using RAB11FIP1 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using RAB11FIP1 mouse mAb with DAB staining.
Flow cytometric
Figure 8:Flow cytometric analysis of Raji cells using RAB11FIP1 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.