RAB11FIP1 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesRCP; NOEL1A; rab11-FIP1
ProductOrderR
Clone#1A12B9D8
Entrez GenelD80223
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human RAB11FIP1 (AA: 130-271) expressed in E. Coli.
MW137.2kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman, Mouse
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
130-271
Catalog#: 30825
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Images
Elisa
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western Blot
Figure 2:Western blot analysis using RAB11FIP1 mAb against human RAB11FIP1 (AA: 130-271) recombinant protein. (Expected MW is 41.7 kDa)
Figure 2:Western blot analysis using RAB11FIP1 mAb against human RAB11FIP1 (AA: 130-271) recombinant protein. (Expected MW is 41.7 kDa)
Western Blot
Figure 3:Western blot analysis using RAB11FIP1 mAb against HEK293 (1) and RAB11FIP1 (AA: 130-271)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using RAB11FIP1 mAb against HEK293 (1) and RAB11FIP1 (AA: 130-271)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using RAB11FIP1 mouse mAb against Raji (1), SW620 (2), A431 (3), SW480 (4), HepG2 (5), Hela (6), and NIH3T3 (7) cell lysate.
Figure 4:Western blot analysis using RAB11FIP1 mouse mAb against Raji (1), SW620 (2), A431 (3), SW480 (4), HepG2 (5), Hela (6), and NIH3T3 (7) cell lysate.
Immunofluorescence analysis
Figure 5:Immunofluorescence analysis of Hela cells using RAB11FIP1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 5:Immunofluorescence analysis of Hela cells using RAB11FIP1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using RAB11FIP1 mouse mAb with DAB staining.
Figure 6:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using RAB11FIP1 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using RAB11FIP1 mouse mAb with DAB staining.
Figure 7:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using RAB11FIP1 mouse mAb with DAB staining.
Flow cytometric
Figure 8:Flow cytometric analysis of Raji cells using RAB11FIP1 mouse mAb (green) and negative control (red).
Figure 8:Flow cytometric analysis of Raji cells using RAB11FIP1 mouse mAb (green) and negative control (red).
Product Overview
DescriptionThis gene encodes one of the Rab11-family interacting proteins (Rab11-FIPs), which play a role in the Rab-11 mediated recycling of vesicles. The encoded protein may be involved in endocytic sorting, trafficking of proteins including integrin subunits and epidermal growth factor receptor (EGFR), and transport between the recycling endosome and the trans-Golgi network. Alternative splicing results in multiple transcript variants. A pseudogene is described on the X chromosome
References (references)
References (references)1.J Clin Invest. 2009 Aug;119(8):2171-83.
2.J Mol Endocrinol. 2005 Oct;35(2):357-72.