PTPN14 Primary Antibody

Item Information
Catalog # Size/Concentration Price
31049 100μl $341.00
Specification
AliasesPEZ; PTP36
Product OrderP
Clone#2C12E3
Entrez GeneID5784
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human PTPN14 (AA: 896-1169) expressed in E. Coli.
MW135.3kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C.  Learn More
Species ReactivityHuman
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
IHC_P (Immunohistochemistry) 1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
896-1169
Catalog
31049
$341.00
-
+

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Images

Elisa

Cell Culture Products
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Cell Culture Products
Figure 2:Western blot analysis using PTPN14 mAb against human PTPN14 (AA: 896-1169) recombinant protein. (Expected MW is 57.5 kDa)

Western Blot

Cell Culture Products
Figure 3:Western blot analysis using PTPN14 mAb against HEK293 (1) and PTPN14 (AA: 896-1169)-hIgGFc transfected HEK293 (2) cell lysate.

Immunofluorescence analysis

Cell Culture Products
Figure 4:Immunofluorescence analysis of Hela cells using PTPN14 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Immunofluorescence analysis

Cell Culture Products
Figure 5:Immunofluorescence analysis of Hela cells using PTPN14 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric

Cell Culture Products
Figure 6:Flow cytometric analysis of Hela cells using PTPN14 mouse mAb (green) and negative control (red).

Immunohistochemical analysis

Cell Culture Products
Figure 7:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using PTPN14 mouse mAb with DAB staining.

Immunohistochemical analysis

Cell Culture Products
Figure 8:Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues using PTPN14 mouse mAb with DAB staining.
Tissue Array Results
Position Tissues Name Result Localization
Nucleus Cytoplasm Membrane
A1/2 Esophageal Cancer +
B1/2 Esophageal Cancer -
C1/2 Esophagus -
D1/2 Esophagus -
E1/2 Stomach Cancer -
F1/2 Stomach Cancer -
G1/2 Stomach +
H1/2 Stomach -
A3/4 Colon Cancer +
B3/4 Colon Cancer +
C3/4 Colon +
D3/4 Duodenum +
E3/4 Rectal Cancer +
F3/4 Rectal Cancer +
G3/4 Rectum -
H3/4 Testis +
A5/6 Hepatic Carcinoma -
B5/6 Hepatic Carcinoma +
C5/6 Liver +
D5/6 Renal cell Carcinoma -
E5/6 Kidney +
F5/6 Pancreas +
G5/6 Prostate Cancer -
H5/6 Lymphoid tissue -
A7/8 Lung Cancer -
C7/8 Lung -
D7/8 Brain -
E7/8 Brain -
F7/8 Medulla oblongata -
G7/8 Cerebellar -
H7/8 Skeletal muscle -
A9/10 Breast Cancer -
B9/10 Breast Cancer +
C9/10 Ovarian Cancer -
D9/10 Ovarian Cancer -
E9/10 Endometrial Cancer +
F9/10 Cervical Cancer -
G9/10 Bladder Cancer -
H9/10 Myocardial -
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Product Overview
Description The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an N-terminal noncatalytic domain similar to that of band 4.1 superfamily cytoskeleton-associated proteins, which suggested the membrane or cytoskeleton localization of this protein. It appears to regulate lymphatic development in mammals, and a loss of function mutation has been found in a kindred with a lymphedema-choanal atresia.
References (references)
References (references) 1.Oncogene. 2013 Apr 18;32(16):2087-95.
2.PLoS One. 2013 Apr 16;8(4):e61916.

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