PTPN1 Primary Antibody

Item Information
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Price
Description

The protein encoded by this gene is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase. This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of this PTP in cell growth control, and cell response to interferon stimulation. Two transcript variants encoding different isoforms have been found for this gene.

Product Overview
Entrez GenelD
5770
Aliases
PTP1B
Clone#
4F8F11
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human PTPN1 (AA: 40-246) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide.
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1. Med Oncol. 2012 Jun;29(2):948-56.
2. Cell Biol Int. 2010 Jul;34(7):747-53.
Product Image
Western Blot
Figure 1: Western blot analysis using PTPN1 mAb against human PTPN1 (AA: 40-246) recombinant protein. (Expected MW is 50 kDa)
Figure 1: Western blot analysis using PTPN1 mAb against human PTPN1 (AA: 40-246) recombinant protein. (Expected MW is 50 kDa)
Western Blot
Figure 2: Western blot analysis using PTPN1 mAb against HEK293 (1) and PTPN1 (AA: 40-246)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 2: Western blot analysis using PTPN1 mAb against HEK293 (1) and PTPN1 (AA: 40-246)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 3: Western blot analysis using PTPN1 mouse mAb against Jurkat (1), MCF-7 (2) cell lysate.
Figure 3: Western blot analysis using PTPN1 mouse mAb against Jurkat (1), MCF-7 (2) cell lysate.
Immunofluorescence analysis
Figure 4:Immunofluorescence analysis of Hela cells using PTPN1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 4:Immunofluorescence analysis of Hela cells using PTPN1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 5: Flow cytometric analysis of Jurkat cells using PTPN1 mouse mAb (green) and negative control (red).
Figure 5: Flow cytometric analysis of Jurkat cells using PTPN1 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 6: Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using PTPN1 mouse mAb with DAB staining.
Figure 6: Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using PTPN1 mouse mAb with DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.