Mouse Monoclonal Antibody to PTH
Item Information
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Price
Description
This gene encodes a member of the parathyroid family of proteins. The encoded preproprotein is proteolytically processed to generate a protein that binds to the parathyroid hormone/parathyroid hormone-related peptide receptor and regulates blood calcium and phosphate levels. Excess production of the encoded protein, known as hyperparathyroidism, can result in hypercalcemia and kidney stones. On the other hand, defective processing of the encoded protein may lead to hypoparathyroidism, which can result in hypocalcemia and numbness. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]
Product Overview
Entrez GenelD
5741
Aliases
FIH1; PTH1
Clone#
8C5H9
Host / Isotype
Mouse / IgG2b
Immunogen
Purified recombinant fragment of human PTH (AA: 32-115) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1，Endocr Metab Immune Disord Drug Targets. 2019;19(8):1134-1140.2，Sci Rep. 2019 Aug 5;9(1):11301.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using PTH mAb against human PTH (AA: 32-115) recombinant protein. (Expected MW is 22 kDa)
Western Blot
Figure 3:Western blot analysis using PTH mAb against HEK293-6e (1) and PTH (AA: 32-115)-hIgGFc transfected HEK293-6e (2) cell lysate.
Flow cytometric analysis
Figure 4:Flow cytometric analysis of Hela cells using PTH mouse mAb (green) and negative control (red).
Flow cytometric analysis
Figure 5:Flow cytometric analysis of HepG2 cells using PTH mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using PTH mouse mAb with DAB staining.
Immunofluorescence analysis
Figure 7:Immunofluorescence analysis of Hela cells using PTH mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
For Research Use Only. Not for use in diagnostic procedures.