Mouse Monoclonal Antibody to PTGS2

Item Information

Catalog #

Size

Price

Description

Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis.

Product Overview

Entrez GenelD

5743

Aliases

COX2; COX-2; PHS-2; PGG/HS; PGHS-2; hCox-2; GRIPGHS

Clone#

4C1H7

Host / Isotype

Mouse / IgG1

Immunogen

Purified recombinant fragment of human PTGS2 (AA: 18-207) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

4℃; -20℃ for long term storage

Product Applications

WB (Western Blot)

1/500 - 1/2000

ICC (Immunocytochemistry)

1/200 - 1/1000

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1,PLoS One. 2020 Sep 30;15(9):e0239856.2,Acta Gastroenterol Belg. Apr-Jun 2020;83(2):249-254.

Product Image

Elisa

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using PTGS2 mAb against human PTGS2 (AA: 18-207) recombinant protein. (Expected MW is 47.8 kDa)

Western Blot

Figure 3:Western blot analysis using PTGS2 mAb against HEK293-6e (1) and human PTGS2 (AA: 18-207)-hIgGFc transfected HEK293-6e (2) cell lysate.

Immunofluorescence analysis

Figure 4:Immunofluorescence analysis of Hela cells using PTGS2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric analysis