PSIP1 Primary Antibody

Item Information

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Price

Description

Transcriptional coactivator involved in neuroepithelial stem cell differentiation and neurogenesis. Involved in particular in lens epithelial cell gene regulation and stress responses. May play an important role in lens epithelial to fiber cell terminal differentiation. May play a protective role during stress-induced apoptosis. Isoform 2 is a more general and stronger transcriptional coactivator. Isoform 2 may also act as an adapter to coordinate pre-mRNA splicing. Cellular cofactor for lentiviral integration. Tissue specificity: Widely expressed. Expressed at high level in the thymus. Expressed in fetal and adult brain. Expressed in neurons, but not astrocytes. Markedly elevated in fetal as compared to adult brain. In the adult brain, expressed in the subventricular zone (SVZ), in hippocampus, and undetectable elsewhere. In the fetal brain, expressed in the germinal neuroepithelium and cortical plate regions.

Product Overview

Entrez GenelD

11168

Aliases

p52; p75; PAIP; DFS70; LEDGF; PSIP2; MGC74712; PSIP1

Clone#

6E4

Host / Isotype

Mouse / IgG1

Species Reactivity

Human, Monkey, Rat

Immunogen

Purified recombinant fragment of human PSIP1 expressed in E. Coli.

Formulation

Ascitic fluid containing 0.03% sodium azide.

Storage

4°C; -20°C for long term storage

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200 - 1/1000

ICC (Immunocytochemistry)

1/200 - 1/1000

ELISA

1/10000

References

1. J Virol. 2008 Dec;82(23):11555-67.
2. Proteins. 2008 Aug;72(2):635-45.

Product Image

Western Blot

Figure 1: Western blot analysis using PSIP1 mouse mAb against HepG2 (1), Jurkat (2), K562 (3), Cos7 (4), PC-12 (5), Hela (6), and NIH/3T3 (7) cell lysate.

Immunohistochemical analysis

Figure 2: Immunohistochemical analysis of paraffin-embedded breast cancer tissues (left) and ovarian cancer tissues (right) using PSIP1 mouse mAb with DAB staining.

Immunohistochemical analysis

Figure 3: Immunohistochemical analysis of paraffin-embedded lung cancer tissues (left) and brain tissues (right) using PSIP1 mouse mAb with DAB staining.

Immunofluorescence analysis

Figure 4: Immunofluorescence analysis of NIH/3T3 cells using PSIP1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

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