Mouse Monoclonal Antibody to PSCA

Item Information
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Description

This gene encodes a glycosylphosphatidylinositol-anchored cell membrane glycoprotein. In addition to being highly expressed in the prostate it is also expressed in the bladder, placenta, colon, kidney, and stomach. This gene is up-regulated in a large proportion of prostate cancers and is also detected in cancers of the bladder and pancreas. This gene includes a polymorphism that results in an upstream start codon in some individuals; this polymorphism is thought to be associated with a risk for certain gastric and bladder cancers. Alternative splicing results in multiple transcript variants.

Product Overview
Entrez GenelD
8000
Aliases
PRO232
Clone#
5H5A7
Host / Isotype
Mouse / IgG2a
Immunogen
Purified recombinant fragment of human PSCA (AA: 1-114) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Biosci Rep. 2019 Sep 3;39(9):BSR20181025. 2.Cancer Prev Res (Phila). 2019 Sep;12(9):579-584.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using PSCA mAb against human PSCA (AA: 1-114) recombinant protein. (Expected MW is 37.8 kDa)
Figure 2:Western blot analysis using PSCA mAb against human PSCA (AA: 1-114) recombinant protein. (Expected MW is 37.8 kDa)
Western Blot
Figure 3:Western blot analysis using PSCA mAb against HEK293-6e (1) and PSCA (AA: 1-114)-hIgGFc transfected HEK293-6e (2) cell lysate.
Figure 3:Western blot analysis using PSCA mAb against HEK293-6e (1) and PSCA (AA: 1-114)-hIgGFc transfected HEK293-6e (2) cell lysate.
Immunofluorescence analysis
Figure 4:Immunofluorescence analysis of Hela cells using PSCA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 4:Immunofluorescence analysis of Hela cells using PSCA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis
Figure 5:Flow cytometric analysis of PC-3 cells using PSCA mouse mAb (green) and negative control (red).
Figure 5:Flow cytometric analysis of PC-3 cells using PSCA mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.