PRL Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
ProductOrderP
Clone#2H10B2
Entrez GenelD5617
FormulationPurified antibody in PBS with 0.05% sodium azide.
HostMouse
IsotypeIgG2b
ImmunogenPurified recombinant fragment of human PRL (AA: 65-173) expressed in E. Coli.
MW26kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
65-173
Catalog#: 30657
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Images
Western Blot
Figure 1: Western blot analysis using PRL mAb against human PRL (AA: 65-173) recombinant protein. (Expected MW is 38.5 kDa)
Figure 1: Western blot analysis using PRL mAb against human PRL (AA: 65-173) recombinant protein. (Expected MW is 38.5 kDa)
Western Blot
Figure 2: Western blot analysis using PRL mAb against HEK293 (1) and PRL (AA: 65-173)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 2: Western blot analysis using PRL mAb against HEK293 (1) and PRL (AA: 65-173)-hIgGFc transfected HEK293 (2) cell lysate.
Immunofluorescence analysis
Figure 3: Immunofluorescence analysis of Hela cells using PRL mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 3: Immunofluorescence analysis of Hela cells using PRL mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Immunofluorescence analysis
Figure 4: Immunofluorescence analysis of MCF-7 cells using PRL mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 4: Immunofluorescence analysis of MCF-7 cells using PRL mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 5: Flow cytometric analysis of Hela cells using PRL mouse mAb (green) and negative control (red).
Figure 5: Flow cytometric analysis of Hela cells using PRL mouse mAb (green) and negative control (red).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Product Overview
Description

This gene encodes the anterior pituitary hormone prolactin. This secreted hormone is a growth regulator for many tissues, including cells of the immune system. It may also play a role in cell survival by suppressing apoptosis, and it is essential for lactation. Alternative splicing results in multiple transcript variants that encode the same protein.

References (references)
References (references)1. J Neuroendocrinol. 2012 Jul;24(7):977-90.
2. Mol Cell Endocrinol. 2012 Jun 5;356(1-2):80-7.