PRL Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene encodes the anterior pituitary hormone prolactin. This secreted hormone is a growth regulator for many tissues, including cells of the immune system. It may also play a role in cell survival by suppressing apoptosis, and it is essential for lactation. Alternative splicing results in multiple transcript variants that encode the same protein.
Product Overview
Entrez GenelD
5617
Clone#
2H10H5
Host / Isotype
Mouse / IgG2b
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human PRL (AA: 65-173) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide.
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1. J Neuroendocrinol. 2012 Jul;24(7):977-90.
2. Mol Cell Endocrinol. 2012 Jun 5;356(1-2):80-7.
2. Mol Cell Endocrinol. 2012 Jun 5;356(1-2):80-7.
Product Image
Western Blot
Figure 1: Western blot analysis using PRL mAb against human PRL (AA: 65-173) recombinant protein. (Expected MW is 38.5 kDa)
Western Blot
Figure 2: Western blot analysis using PRL mAb against HEK293 (1) and PRL (AA: 65-173)-hIgGFc transfected HEK293 (2) cell lysate.
Immunofluorescence analysis
Figure 3: Immunofluorescence analysis of Hela cells using PRL mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 4: Flow cytometric analysis of Hela cells using PRL mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 5: Immunohistochemical analysis of paraffin-embedded muscle tissues using PRL mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 6: Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using PRL mouse mAb with DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.