PRKAG3 Primary Antibody
Item Information
Catalog #
Size
Price
Description
The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK. It is dominantly expressed in skeletal muscle. Studies of the pig counterpart suggest that this subunit may play a key role in the regulation of energy metabolism in skeletal muscle.
Product Overview
Entrez GenelD
53632
Aliases
AMPKG3
Clone#
2C7A7
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human PRKAG3 (AA: 9-151) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
ELISA
1/10000
References
1.Diabetologia. 2010 Sep;53(9):1986-97.
2.PLoS One. 2007 Sep 19;2(9):e903.
2.PLoS One. 2007 Sep 19;2(9):e903.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using PRKAG3 mAb against human PRKAG3 (AA: 9-151) recombinant protein. (Expected MW is 41.1 kDa)
Western Blot
Figure 3:Western blot analysis using PRKAG3 mAb against HEK293 (1) and PRKAG3 (AA: 9-151)-hIgGFc transfected HEK293 (2) cell lysate.
Immunofluorescence analysis
Figure 4:Immunofluorescence analysis of Hela cells using PRKAG3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Immunohistochemical analysis
Figure 5:Immunohistochemical analysis of paraffin-embedded rectum tissues using PRKAG3 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.