PRKAB2 Primary Antibody
The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. It is highly expressed in skeletal muscle and thus may have tissue-specific roles. Multiple alternatively spliced transcript variants have been found for this gene.
Purified recombinant fragment of human PRKAB2 (AA: 1-120) expressed in E. Coli.
Purified antibody in PBS with 0.05% sodium azide
4°C; -20°C for long term storage
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 2:Western blot analysis using PRKAB2 mAb against human PRKAB2 (AA: 1-120) recombinant protein. (Expected MW is 39 kDa)
Figure 3:Western blot analysis using PRKAB2 mAb against HEK293 (1) and PRKAB2 (AA: 1-120)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Flow cytometric analysis of Hela cells using PRKAB2 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.