PRKAA2 Primary Antibody
The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.
Purified antibody in PBS with 0.05% sodium azide
4°C; -20°C for long term storage
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 2:Western blot analysis using PRKAA2 mAb against human *** (AA: 453-552) recombinant protein. (Expected MW is 36.7 kDa)
Figure 3:Western blot analysis using PRKAA2 mAb against HEK293 (1) and PRKAA2 (AA: 453-552)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Western blot analysis using PRKAA2 mouse mAb against HEK293 (1) and COS7 (2) cell lysate.
Figure 5:Flow cytometric analysis of Jurkat cells using PRKAA2 mouse mAb (green) and negative control (red).
Figure 6:Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using PRKAA2 mouse mAb with DAB staining.
Figure 7:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using PRKAA2 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.