PRKAA2 Primary Antibody
Item Information
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Size
Price
Description
The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.
Product Overview
Entrez GenelD
5563
Aliases
AMPK; AMPK2; PRKAA; AMPKa2
Clone#
8E11H5
Host / Isotype
Mouse / IgG1
Species Reactivity
Human, Monkey
Immunogen
Purified recombinant fragment of human PRKAA2 (AA: 453-552) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Pathobiology. 2015;82(5):203-11.
2.Acta Crystallogr D Biol Crystallogr. 2011 May;67(Pt 5):480-7.
2.Acta Crystallogr D Biol Crystallogr. 2011 May;67(Pt 5):480-7.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using PRKAA2 mAb against human *** (AA: 453-552) recombinant protein. (Expected MW is 36.7 kDa)
Western Blot
Figure 3:Western blot analysis using PRKAA2 mAb against HEK293 (1) and PRKAA2 (AA: 453-552)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using PRKAA2 mouse mAb against HEK293 (1) and COS7 (2) cell lysate.
Flow cytometric
Figure 5:Flow cytometric analysis of Jurkat cells using PRKAA2 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using PRKAA2 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using PRKAA2 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.