PRDM4 Primary Antibody
Item Information
Catalog #
Size
Price
Description
The protein encoded by this gene is a transcription factor of the PR-domain protein family. It contains a PR-domain and multiple zinc finger motifs. Transcription factors of the PR-domain family are known to be involved in cell differentiation and tumorigenesis. An elevated expression level of this gene has been observed in PC12 cells treated with nerve growth factor, beta polypeptide (NGF). This gene is located in a chromosomal region that is thought to contain tumor suppressor genes.
Product Overview
Entrez GenelD
11108
Aliases
PFM1
Clone#
4B6E8
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human PRDM4 (AA: 476-575) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.J Biol Chem. 2012 Dec 14;287(51):42995-3006.
2.Proteins. 2011 Jul;79(7):2341-5.
2.Proteins. 2011 Jul;79(7):2341-5.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western Blot
Figure 2:Western blot analysis using PRDM4 mAb against human PRDM4 (AA: 476-575) recombinant protein. (Expected MW is 37.7 kDa)
Western Blot
Figure 3:Western blot analysis using PRDM4 mAb against HEK293 (1) and PRDM4 (AA: 476-575)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 4:Flow cytometric analysis of HeLa cells using PRDM4 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 5:Immunohistochemical analysis of paraffin-embedded liver cancer tissues using PRDM4 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues using PRDM4 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.