PRDM14 Primary Antibody

Item Information
Catalog #
Size
Price
Description
This gene encodes a member of the PRDI-BF1 and RIZ homology domain containing (PRDM) family of transcriptional regulators. The encoded protein may possess histone methyltransferase activity and plays a critical role in cell pluripotency by suppressing the expression of differentiation marker genes. Expression of this gene may play a role in breast cancer.
Product Overview
Entrez GenelD
63978
Aliases
PFM11
Clone#
4C8B6
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human PRDM14 (AA: 4-203) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Carcinogenesis. 2014 Nov;35(11):2611-8.
2.Med Oncol. 2013;30(3):605.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using PRDM14 mAb against human PRDM14 (AA: 4-203) recombinant protein. (Expected MW is 50.3 kDa)
Figure 2:Western blot analysis using PRDM14 mAb against human PRDM14 (AA: 4-203) recombinant protein. (Expected MW is 50.3 kDa)
Western Blot
Figure 3:Western blot analysis using PRDM14 mAb against HEK293 (1) and PRDM14 (AA: 4-203)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using PRDM14 mAb against HEK293 (1) and PRDM14 (AA: 4-203)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 5:Flow cytometric analysis of A549 cells using PRDM14 mouse mAb (green) and negative control (red).
Figure 5:Flow cytometric analysis of A549 cells using PRDM14 mouse mAb (green) and negative control (red).
Western Blot
Figure 6:Western blot analysis using *** mouse mAb against HEK293 (1), A549 (2), and HepG2 (3) cell lysate.
Figure 6:Western blot analysis using *** mouse mAb against HEK293 (1), A549 (2), and HepG2 (3) cell lysate.
For Research Use Only. Not for use in diagnostic procedures.